000			03012nam a22002777a 4500
003			phtghnu
005			20250228143724.0
007			ta
008			250228b2021 ph ||||| |||| 00| 0 eng d
040			_beng _cHNU _erda
082			_223 _3Th _a579.135 P13 _b2021
100			_aPadiera, Karl B., _eauthor.
245			_aUse of Allium cepa, Allium sativum, Ananas comosus, and Musa acuminata peels as an alternative fungal media / _cKarl B. Padiera, RMT, MSMT; Nino T. Majerano RMT, Darwin L. Cruz, RMT.
264			_a[Tagbilaran City, Bohol] : _b[Holy Name University], _c[2021].
300			_axli, 78 pages ; _c28 cm.
504			_aIncludes bibliographical references.
520			_aThis study aimed to determine the biochemical components of garlic, onion, pineapple and banana peels as well as compare the fungal growth of Cladosporium spp., Trichophyton spp., and Fusarium spp. using the experimental culture media derived from garlic, onion, pineapple ad banana peels. The vegetable and fruit peels were collected from wet markets, dried, pulverized, sieved, and were stored in a clean screw-cap container. The pulverized peels were used as the experimental culture media. Fungi were cultured in the four pure experimental culture media and in the seven mixed experimental culture media. The growth of fungi was also compared against the positive control media particularly the Sabouraud Dextrose Agar (SDA) and Nutrient Agar (NA). All cultures were incubated at toom temperature (28oC) for 3 days. The fungal growth was measured by using the Vernier caliper. Results from biochemical tests revealed the presence of carbohydrates and sulfur for all vegetable and fruit filtrates while phosphorus was only present in banana peel filtrate. The pH of the experimental media was 7.0. Cladosporium spp. exhibited the wildest diameter with 26.1 millimeters in the garlic medium, Trichophyton spp. in the onion medium with 34.9 millimeters ad Fusarium spp. in the banana medium with 45.7 millimeters. The growth of Cladosporium spp. and Trichophyton spp. in the mixed experimental culture medium B+P+G+O showed no significant difference with Sabouraud Dextrose Agar and with p-values of 0.522 and 1.64. However, the growth of Fusarium spp. in the Sabouraud Dextrose Agar and the experimental culture medium B+P+G+O demonstrated significant difference with a p-value of 0.007. The findings of this study suggest that formulated mixed experimental culture medium composed of banana, pineapple, garlic, and onion can be an alternative fungal culture medium for the isolation of Cladosporium spp., Trichophyton spp. and Fusarium spp.
521			_aCHS _bBachelor of Science in Medical Technology
546			_aIn English
650			_aMicrobiology (Fungi & Bacteria).
650			_aMedicinal plants & natural products.
700			_aMajerano , Nino T., _eauthor.
700			_a Cruz, Darwin L., _eauthor.
942			_2ddc _cTH _h500-599
999			_c137235 _d137235